diff --git a/cli/flags.rs b/cli/flags.rs
index 6d853860a9..cdd90fa331 100644
--- a/cli/flags.rs
+++ b/cli/flags.rs
@@ -12,6 +12,7 @@ use deno_runtime::permissions::PermissionsOptions;
 use log::debug;
 use log::Level;
 use once_cell::sync::Lazy;
+use std::env;
 use std::net::SocketAddr;
 use std::num::NonZeroU32;
 use std::num::NonZeroU8;
@@ -474,7 +475,9 @@ static ENV_VARIABLES_HELP: &str = r#"ENVIRONMENT VARIABLES:
     DENO_DIR             Set the cache directory
     DENO_INSTALL_ROOT    Set deno install's output directory
                          (defaults to $HOME/.deno/bin)
-    DENO_FUTURE_CHECK    Opt-in to the upcoming behavior of the `deno run` 
+    DENO_NO_PROMPT       Set to disable permission prompts on access
+                         (alternative to passing --no-prompt on invocation)
+    DENO_FUTURE_CHECK    Opt-in to the upcoming behavior of the `deno run`
                          subcommand that doesn't perform type-checking by default.
     DENO_WEBGPU_TRACE    Directory to use for wgpu traces
     HTTP_PROXY           Proxy address for HTTP requests
@@ -2705,7 +2708,11 @@ fn permission_args_parse(flags: &mut Flags, matches: &clap::ArgMatches) {
     flags.allow_ffi = Some(vec![]);
     flags.allow_hrtime = true;
   }
-  if matches.is_present("no-prompt") {
+  #[cfg(not(test))]
+  let has_no_prompt_env = env::var("DENO_NO_PROMPT") == Ok("1".to_string());
+  #[cfg(test)]
+  let has_no_prompt_env = false;
+  if has_no_prompt_env || matches.is_present("no-prompt") {
     flags.no_prompt = true;
   }
 }
diff --git a/cli/tests/integration/bench_tests.rs b/cli/tests/integration/bench_tests.rs
index 7b4fbb0a58..e889a82246 100644
--- a/cli/tests/integration/bench_tests.rs
+++ b/cli/tests/integration/bench_tests.rs
@@ -130,13 +130,14 @@ itest!(filter {
 });
 
 itest!(no_prompt_by_default {
-  args: "bench --unstable bench/no_prompt_by_default.ts",
+  args: "bench --quiet --unstable bench/no_prompt_by_default.ts",
   exit_code: 1,
   output: "bench/no_prompt_by_default.out",
 });
 
 itest!(no_prompt_with_denied_perms {
-  args: "bench --unstable --allow-read bench/no_prompt_with_denied_perms.ts",
+  args:
+    "bench --quiet --unstable --allow-read bench/no_prompt_with_denied_perms.ts",
   exit_code: 1,
   output: "bench/no_prompt_with_denied_perms.out",
 });
diff --git a/cli/tests/integration/run_tests.rs b/cli/tests/integration/run_tests.rs
index 0953e72395..b99ab28906 100644
--- a/cli/tests/integration/run_tests.rs
+++ b/cli/tests/integration/run_tests.rs
@@ -2733,3 +2733,23 @@ itest!(js_root_with_ts_check {
   output: "js_root_with_ts_check.js.out",
   exit_code: 1,
 });
+
+itest!(no_prompt_flag {
+  args: "run --quiet --unstable --no-prompt no_prompt.ts",
+  output_str: Some(""),
+});
+
+#[test]
+fn deno_no_prompt_environment_variable() {
+  let output = util::deno_cmd()
+    .current_dir(util::testdata_path())
+    .arg("run")
+    .arg("--unstable")
+    .arg("no_prompt.ts")
+    .env("DENO_NO_PROMPT", "1")
+    .spawn()
+    .unwrap()
+    .wait_with_output()
+    .unwrap();
+  assert!(output.status.success());
+}
diff --git a/cli/tests/integration/test_tests.rs b/cli/tests/integration/test_tests.rs
index bac50f16dd..3e2df91133 100644
--- a/cli/tests/integration/test_tests.rs
+++ b/cli/tests/integration/test_tests.rs
@@ -288,13 +288,13 @@ itest!(steps_output_within {
 });
 
 itest!(no_prompt_by_default {
-  args: "test test/no_prompt_by_default.ts",
+  args: "test --quiet test/no_prompt_by_default.ts",
   exit_code: 1,
   output: "test/no_prompt_by_default.out",
 });
 
 itest!(no_prompt_with_denied_perms {
-  args: "test --allow-read test/no_prompt_with_denied_perms.ts",
+  args: "test --quiet --allow-read test/no_prompt_with_denied_perms.ts",
   exit_code: 1,
   output: "test/no_prompt_with_denied_perms.out",
 });
diff --git a/cli/tests/testdata/bench/no_prompt_by_default.out b/cli/tests/testdata/bench/no_prompt_by_default.out
index 3fe67b720d..d47198d852 100644
--- a/cli/tests/testdata/bench/no_prompt_by_default.out
+++ b/cli/tests/testdata/bench/no_prompt_by_default.out
@@ -1,4 +1,3 @@
-Check [WILDCARD]no_prompt_by_default.ts
 running 1 bench from [WILDCARD]no_prompt_by_default.ts
 bench no prompt ... 1000 iterations FAILED ([WILDCARD]ms)
 
diff --git a/cli/tests/testdata/bench/no_prompt_with_denied_perms.out b/cli/tests/testdata/bench/no_prompt_with_denied_perms.out
index f6aec6226c..efe9fa6dc9 100644
--- a/cli/tests/testdata/bench/no_prompt_with_denied_perms.out
+++ b/cli/tests/testdata/bench/no_prompt_with_denied_perms.out
@@ -1,4 +1,3 @@
-Check [WILDCARD]/no_prompt_with_denied_perms.ts
 running 1 bench from [WILDCARD]/no_prompt_with_denied_perms.ts
 bench no prompt ... 1000 iterations FAILED ([WILDCARD]ms)
 
diff --git a/cli/tests/testdata/no_prompt.ts b/cli/tests/testdata/no_prompt.ts
new file mode 100644
index 0000000000..88dccc458b
--- /dev/null
+++ b/cli/tests/testdata/no_prompt.ts
@@ -0,0 +1,10 @@
+new Worker("data:,setTimeout(() => Deno.exit(2), 200)", {
+  type: "module",
+  deno: { namespace: true },
+});
+
+try {
+  await Deno.run({ cmd: ["ps"] });
+} catch {
+  Deno.exit(0);
+}
diff --git a/cli/tests/testdata/test/no_prompt_by_default.out b/cli/tests/testdata/test/no_prompt_by_default.out
index 705aca6891..61fcea8c8d 100644
--- a/cli/tests/testdata/test/no_prompt_by_default.out
+++ b/cli/tests/testdata/test/no_prompt_by_default.out
@@ -1,4 +1,3 @@
-Check [WILDCARD]/no_prompt_by_default.ts
 running 1 test from ./test/no_prompt_by_default.ts
 no prompt ... FAILED ([WILDCARD]ms)
 
diff --git a/cli/tests/testdata/test/no_prompt_with_denied_perms.out b/cli/tests/testdata/test/no_prompt_with_denied_perms.out
index d80fb304c6..16a2092c54 100644
--- a/cli/tests/testdata/test/no_prompt_with_denied_perms.out
+++ b/cli/tests/testdata/test/no_prompt_with_denied_perms.out
@@ -1,4 +1,3 @@
-Check [WILDCARD]/no_prompt_with_denied_perms.ts
 running 1 test from ./test/no_prompt_with_denied_perms.ts
 no prompt ... FAILED ([WILDCARD]ms)