diff --git a/cli/args/mod.rs b/cli/args/mod.rs
index e0eff6171d..553af51a17 100644
--- a/cli/args/mod.rs
+++ b/cli/args/mod.rs
@@ -793,19 +793,7 @@ impl CliOptions {
     )
     .with_context(|| "Resolving node_modules folder.")?;
 
-    if let Some(env_file_name) = &flags.env_file {
-      match from_filename(env_file_name) {
-          Ok(_) => (),
-          Err(error) => {
-            match error {
-              dotenvy::Error::LineParse(line, index)=> log::info!("{} Parsing failed within the specified environment file: {} at index: {} of the value: {}",colors::yellow("Warning"), env_file_name, index, line),
-              dotenvy::Error::Io(_)=> log::info!("{} The `--env` flag was used, but the environment file specified '{}' was not found.",colors::yellow("Warning"),env_file_name),
-              dotenvy::Error::EnvVar(_)=>log::info!("{} One or more of the environment variables isn't present or not unicode within the specified environment file: {}",colors::yellow("Warning"),env_file_name),
-              _ => log::info!("{} Unknown failure occurred with the specified environment file: {}", colors::yellow("Warning"), env_file_name),
-            }
-          }
-        }
-    }
+    load_env_variables_from_env_file(flags.env_file.as_ref());
 
     let disable_deprecated_api_warning = flags.log_level
       == Some(log::Level::Error)
@@ -1118,6 +1106,10 @@ impl CliOptions {
     }
   }
 
+  pub fn env_file_name(&self) -> Option<&String> {
+    self.flags.env_file.as_ref()
+  }
+
   pub fn enable_future_features(&self) -> bool {
     *DENO_FUTURE
   }
@@ -1871,6 +1863,23 @@ pub fn config_to_deno_graph_workspace_member(
   })
 }
 
+fn load_env_variables_from_env_file(filename: Option<&String>) {
+  let Some(env_file_name) = filename else {
+    return;
+  };
+  match from_filename(env_file_name) {
+    Ok(_) => (),
+    Err(error) => {
+      match error {
+          dotenvy::Error::LineParse(line, index)=> log::info!("{} Parsing failed within the specified environment file: {} at index: {} of the value: {}",colors::yellow("Warning"), env_file_name, index, line),
+          dotenvy::Error::Io(_)=> log::info!("{} The `--env` flag was used, but the environment file specified '{}' was not found.",colors::yellow("Warning"),env_file_name),
+          dotenvy::Error::EnvVar(_)=> log::info!("{} One or more of the environment variables isn't present or not unicode within the specified environment file: {}",colors::yellow("Warning"),env_file_name),
+          _ => log::info!("{} Unknown failure occurred with the specified environment file: {}", colors::yellow("Warning"), env_file_name),
+        }
+    }
+  }
+}
+
 #[cfg(test)]
 mod test {
   use pretty_assertions::assert_eq;
diff --git a/cli/mainrt.rs b/cli/mainrt.rs
index 6a363c04b5..d4f0f558ea 100644
--- a/cli/mainrt.rs
+++ b/cli/mainrt.rs
@@ -31,6 +31,7 @@ pub use deno_runtime::UNSTABLE_GRANULAR_FLAGS;
 use deno_terminal::colors;
 
 use std::borrow::Cow;
+use std::collections::HashMap;
 use std::env;
 use std::env::current_exe;
 
@@ -70,6 +71,14 @@ fn unwrap_or_exit<T>(result: Result<T, AnyError>) -> T {
   }
 }
 
+fn load_env_vars(env_vars: &HashMap<String, String>) {
+  env_vars.iter().for_each(|env_var| {
+    if env::var(env_var.0).is_err() {
+      std::env::set_var(env_var.0, env_var.1);
+    }
+  })
+}
+
 fn main() {
   let args: Vec<_> = env::args_os().collect();
   let current_exe_path = current_exe().unwrap();
@@ -79,6 +88,8 @@ fn main() {
     match standalone {
       Ok(Some(future)) => {
         let (metadata, eszip) = future.await?;
+        util::logger::init(metadata.log_level);
+        load_env_vars(&metadata.env_vars_from_env_file);
         let exit_code = standalone::run(eszip, metadata).await?;
         std::process::exit(exit_code);
       }
diff --git a/cli/standalone/binary.rs b/cli/standalone/binary.rs
index c9371d853e..4d6b32b7df 100644
--- a/cli/standalone/binary.rs
+++ b/cli/standalone/binary.rs
@@ -2,6 +2,7 @@
 
 use std::borrow::Cow;
 use std::collections::BTreeMap;
+use std::collections::HashMap;
 use std::collections::VecDeque;
 use std::env::current_exe;
 use std::ffi::OsString;
@@ -96,6 +97,7 @@ pub struct Metadata {
   pub ca_stores: Option<Vec<String>>,
   pub ca_data: Option<Vec<u8>>,
   pub unsafely_ignore_certificate_errors: Option<Vec<String>>,
+  pub env_vars_from_env_file: HashMap<String, String>,
   pub workspace_resolver: SerializedWorkspaceResolver,
   pub entrypoint_key: String,
   pub node_modules: Option<NodeModules>,
@@ -584,6 +586,14 @@ impl<'a> DenoCompileBinaryWriter<'a> {
       }
     };
 
+    let env_vars_from_env_file = match cli_options.env_file_name() {
+      Some(env_filename) => {
+        log::info!("{} Environment variables from the file \"{}\" were embedded in the generated executable file", crate::colors::yellow("Warning"), env_filename);
+        get_file_env_vars(env_filename.to_string())?
+      }
+      None => Default::default(),
+    };
+
     let metadata = Metadata {
       argv: compile_flags.args.clone(),
       seed: cli_options.seed(),
@@ -596,6 +606,7 @@ impl<'a> DenoCompileBinaryWriter<'a> {
       log_level: cli_options.log_level(),
       ca_stores: cli_options.ca_stores().clone(),
       ca_data,
+      env_vars_from_env_file,
       entrypoint_key: root_dir_url.specifier_key(entrypoint).into_owned(),
       workspace_resolver: SerializedWorkspaceResolver {
         import_map: self.workspace_resolver.maybe_import_map().map(|i| {
@@ -757,6 +768,21 @@ impl<'a> DenoCompileBinaryWriter<'a> {
   }
 }
 
+/// This function returns the environment variables specified
+/// in the passed environment file.
+fn get_file_env_vars(
+  filename: String,
+) -> Result<HashMap<String, String>, dotenvy::Error> {
+  let mut file_env_vars = HashMap::new();
+  for item in dotenvy::from_filename_iter(filename)? {
+    let Ok((key, val)) = item else {
+      continue; // this failure will be warned about on load
+    };
+    file_env_vars.insert(key, val);
+  }
+  Ok(file_env_vars)
+}
+
 /// This function sets the subsystem field in the PE header to 2 (GUI subsystem)
 /// For more information about the PE header: https://learn.microsoft.com/en-us/windows/win32/debug/pe-format
 fn set_windows_binary_to_gui(bin: &mut [u8]) -> Result<(), AnyError> {
diff --git a/tests/specs/compile/env_vars_support/__test__.jsonc b/tests/specs/compile/env_vars_support/__test__.jsonc
new file mode 100644
index 0000000000..4fc80e1e4d
--- /dev/null
+++ b/tests/specs/compile/env_vars_support/__test__.jsonc
@@ -0,0 +1,14 @@
+{
+  "tempDir": true,
+  "steps": [
+    {
+      "args": "compile -A --output out --env=environment.env main.ts",
+      "output": "compile.out"
+    },
+    {
+      "commandName": "./out",
+      "args": [],
+      "output": "main.out"
+    }
+  ]
+}
diff --git a/tests/specs/compile/env_vars_support/compile.out b/tests/specs/compile/env_vars_support/compile.out
new file mode 100644
index 0000000000..2d004e7cb1
--- /dev/null
+++ b/tests/specs/compile/env_vars_support/compile.out
@@ -0,0 +1,4 @@
+Warning Parsing failed within the specified environment file: environment.env at index: 3 of the value: c:\path
+Check [WILDCARD]main.ts
+Compile [WILDCARD]main.ts to out[WILDCARD]
+Warning Environment variables from the file "environment.env" were embedded in the generated executable file
diff --git a/tests/specs/compile/env_vars_support/environment.env b/tests/specs/compile/env_vars_support/environment.env
new file mode 100644
index 0000000000..3da867c500
--- /dev/null
+++ b/tests/specs/compile/env_vars_support/environment.env
@@ -0,0 +1,4 @@
+FOO=valid
+MULTILINE="First Line
+Second Line"
+ANOTHER_FOO=c:\path
\ No newline at end of file
diff --git a/tests/specs/compile/env_vars_support/main.out b/tests/specs/compile/env_vars_support/main.out
new file mode 100644
index 0000000000..f8f0130abb
--- /dev/null
+++ b/tests/specs/compile/env_vars_support/main.out
@@ -0,0 +1,4 @@
+valid
+undefined
+First Line
+Second Line
diff --git a/tests/specs/compile/env_vars_support/main.ts b/tests/specs/compile/env_vars_support/main.ts
new file mode 100644
index 0000000000..48488ce721
--- /dev/null
+++ b/tests/specs/compile/env_vars_support/main.ts
@@ -0,0 +1,3 @@
+console.log(Deno.env.get("FOO"));
+console.log(Deno.env.get("ANOTHER_FOO"));
+console.log(Deno.env.get("MULTILINE"));